Diminished exoproteome of Frankia spp. in culture and symbiosis.
Identifieur interne : 000252 ( Main/Exploration ); précédent : 000251; suivant : 000253Diminished exoproteome of Frankia spp. in culture and symbiosis.
Auteurs : J E Mastronunzio [États-Unis] ; Y. Huang ; D R BensonSource :
- Applied and environmental microbiology [ 1098-5336 ] ; 2009.
Descripteurs français
- KwdFr :
- Alnus (microbiologie), Chromatographie en phase liquide (MeSH), Elaeagnaceae (microbiologie), Fougères (microbiologie), Frankia (composition chimique), Frankia (croissance et développement), Frankia (physiologie), Protéines bactériennes (analyse), Protéome (analyse), Racines de plante (microbiologie), Spectrométrie de masse en tandem (MeSH), Symbiose (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
- analyse : Protéines bactériennes, Protéome.
- composition chimique : Frankia.
- croissance et développement : Frankia.
- microbiologie : Alnus, Elaeagnaceae, Fougères, Racines de plante.
- physiologie : Frankia.
- Chromatographie en phase liquide, Spectrométrie de masse en tandem, Symbiose, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Alnus (microbiology), Bacterial Proteins (analysis), Chromatography, Liquid (MeSH), Elaeagnaceae (microbiology), Electrophoresis, Gel, Two-Dimensional (MeSH), Ferns (microbiology), Frankia (chemistry), Frankia (growth & development), Frankia (physiology), Plant Roots (microbiology), Proteome (analysis), Symbiosis (MeSH), Tandem Mass Spectrometry (MeSH).
- MESH :
- chemical , analysis : Bacterial Proteins, Proteome.
- chemistry : Frankia.
- growth & development : Frankia.
- microbiology : Alnus, Elaeagnaceae, Ferns, Plant Roots.
- physiology : Frankia.
- Chromatography, Liquid, Electrophoresis, Gel, Two-Dimensional, Symbiosis, Tandem Mass Spectrometry.
Abstract
Frankia species are the most geographically widespread gram-positive plant symbionts, carrying out N(2) fixation in root nodules of trees and woody shrubs called actinorhizal plants. Taking advantage of the sequencing of three Frankia genomes, proteomics techniques were used to investigate the population of extracellular proteins (the exoproteome) from Frankia, some of which potentially mediate host-microbe interactions. Initial two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of culture supernatants indicated that cytoplasmic proteins appeared in supernatants as cells aged, likely because older hyphae lyse in this slow-growing filamentous actinomycete. Using liquid chromatography coupled to tandem mass spectrometry to identify peptides, 38 proteins were identified in the culture supernatant of Frankia sp. strain CcI3, but only three had predicted export signal peptides. In symbiotic cells, 42 signal peptide-containing proteins were detected from strain CcI3 in Casuarina cunninghamiana and Casuarina glauca root nodules, while 73 and 53 putative secreted proteins containing signal peptides were identified from Frankia strains in field-collected root nodules of Alnus incana and Elaeagnus angustifolia, respectively. Solute-binding proteins were the most commonly identified secreted proteins in symbiosis, particularly those predicted to bind branched-chain amino acids and peptides. These direct proteomics results complement a previous bioinformatics study that predicted few secreted hydrolytic enzymes in the Frankia proteome and provide direct evidence that the symbiosis succeeds partly, if not largely, because of a benign relationship.
DOI: 10.1128/AEM.01559-09
PubMed: 19749056
PubMed Central: PMC2772416
Affiliations:
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Huang</name></author><author><name sortKey="Benson, D R" sort="Benson, D R" uniqKey="Benson D" first="D R" last="Benson">D R Benson</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="eISSN">1098-5336</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alnus (microbiology)</term><term>Bacterial Proteins (analysis)</term><term>Chromatography, Liquid (MeSH)</term><term>Elaeagnaceae (microbiology)</term><term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term><term>Ferns (microbiology)</term><term>Frankia (chemistry)</term><term>Frankia (growth & development)</term><term>Frankia (physiology)</term><term>Plant Roots (microbiology)</term><term>Proteome (analysis)</term><term>Symbiosis (MeSH)</term><term>Tandem Mass Spectrometry (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alnus (microbiologie)</term><term>Chromatographie en phase liquide (MeSH)</term><term>Elaeagnaceae (microbiologie)</term><term>Fougères (microbiologie)</term><term>Frankia (composition chimique)</term><term>Frankia (croissance et développement)</term><term>Frankia (physiologie)</term><term>Protéines bactériennes (analyse)</term><term>Protéome (analyse)</term><term>Racines de plante (microbiologie)</term><term>Spectrométrie de masse en tandem (MeSH)</term><term>Symbiose (MeSH)</term><term>Électrophorèse bidimensionnelle sur gel (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Bacterial Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines bactériennes</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Frankia</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Frankia</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Frankia</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Frankia</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Alnus</term><term>Elaeagnaceae</term><term>Fougères</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Alnus</term><term>Elaeagnaceae</term><term>Ferns</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Frankia</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Frankia</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, Liquid</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Symbiosis</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie en phase liquide</term><term>Spectrométrie de masse en tandem</term><term>Symbiose</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Frankia species are the most geographically widespread gram-positive plant symbionts, carrying out N(2) fixation in root nodules of trees and woody shrubs called actinorhizal plants. Taking advantage of the sequencing of three Frankia genomes, proteomics techniques were used to investigate the population of extracellular proteins (the exoproteome) from Frankia, some of which potentially mediate host-microbe interactions. Initial two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of culture supernatants indicated that cytoplasmic proteins appeared in supernatants as cells aged, likely because older hyphae lyse in this slow-growing filamentous actinomycete. Using liquid chromatography coupled to tandem mass spectrometry to identify peptides, 38 proteins were identified in the culture supernatant of Frankia sp. strain CcI3, but only three had predicted export signal peptides. In symbiotic cells, 42 signal peptide-containing proteins were detected from strain CcI3 in Casuarina cunninghamiana and Casuarina glauca root nodules, while 73 and 53 putative secreted proteins containing signal peptides were identified from Frankia strains in field-collected root nodules of Alnus incana and Elaeagnus angustifolia, respectively. Solute-binding proteins were the most commonly identified secreted proteins in symbiosis, particularly those predicted to bind branched-chain amino acids and peptides. These direct proteomics results complement a previous bioinformatics study that predicted few secreted hydrolytic enzymes in the Frankia proteome and provide direct evidence that the symbiosis succeeds partly, if not largely, because of a benign relationship.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19749056</PMID><DateCompleted><Year>2010</Year><Month>02</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-5336</ISSN><JournalIssue CitedMedium="Internet"><Volume>75</Volume><Issue>21</Issue><PubDate><Year>2009</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Diminished exoproteome of Frankia spp. in culture and symbiosis.</ArticleTitle><Pagination><MedlinePgn>6721-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/AEM.01559-09</ELocationID><Abstract><AbstractText>Frankia species are the most geographically widespread gram-positive plant symbionts, carrying out N(2) fixation in root nodules of trees and woody shrubs called actinorhizal plants. Taking advantage of the sequencing of three Frankia genomes, proteomics techniques were used to investigate the population of extracellular proteins (the exoproteome) from Frankia, some of which potentially mediate host-microbe interactions. Initial two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of culture supernatants indicated that cytoplasmic proteins appeared in supernatants as cells aged, likely because older hyphae lyse in this slow-growing filamentous actinomycete. Using liquid chromatography coupled to tandem mass spectrometry to identify peptides, 38 proteins were identified in the culture supernatant of Frankia sp. strain CcI3, but only three had predicted export signal peptides. In symbiotic cells, 42 signal peptide-containing proteins were detected from strain CcI3 in Casuarina cunninghamiana and Casuarina glauca root nodules, while 73 and 53 putative secreted proteins containing signal peptides were identified from Frankia strains in field-collected root nodules of Alnus incana and Elaeagnus angustifolia, respectively. Solute-binding proteins were the most commonly identified secreted proteins in symbiosis, particularly those predicted to bind branched-chain amino acids and peptides. These direct proteomics results complement a previous bioinformatics study that predicted few secreted hydrolytic enzymes in the Frankia proteome and provide direct evidence that the symbiosis succeeds partly, if not largely, because of a benign relationship.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mastronunzio</LastName><ForeName>J E</ForeName><Initials>JE</Initials><AffiliationInfo><Affiliation>Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Rd., Unit 3125, Storrs, CT 06269, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Benson</LastName><ForeName>D R</ForeName><Initials>DR</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, 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uniqKey="Huang Y" first="Y" last="Huang">Y. Huang</name></noCountry><country name="États-Unis"><region name="Connecticut"><name sortKey="Mastronunzio, J E" sort="Mastronunzio, J E" uniqKey="Mastronunzio J" first="J E" last="Mastronunzio">J E Mastronunzio</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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